Nope. And in my opinion this is pretty much a waste of time because:
1) People can run virtual machines on the same PC.
2) People can run L2 from more than 1 computer.
Adding protection about this on the server is basically the same as telling players they can’t have lan parties (cause you’ll be doing some checks on IPs). We won’t be that strict about dualboxing, but we will be strict about botting.
Client side security can be easily bypassed most times, and I don’t dig much into them.